| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Czerwiec, Jesse D (13)
|
MDCBAC |
29.60 |
28.46 |
-1.14 |
| 2 |
Mazzella, Nicholas (14)
|
MDCAC |
30.55 |
28.77 |
-1.78 |
| 3 |
Castillo, Julio J (13)
|
MDCAC |
29.77 |
28.96 |
-0.81 |
| 4 |
Kohl, Sebastian R (14)
|
MDCAC |
30.88 |
29.06 |
-1.82 |
| 5 |
Dorn, Liev E (13)
|
MDCAC |
32.56 |
29.32 |
-3.24 |
| 6 |
Schlee, Tanner R (14)
|
MDCAA |
30.70 |
30.32 |
-0.38 |
| 7 |
Hurbon, Luca C (13)
|
MDCAA |
32.01 |
30.36 |
-1.65 |
| 8 |
Bridges, Thomas (13)
|
MDBAY |
26.70 |
30.39 |
3.69 |
| 9 |
Alleva, Dominic J (14)
|
MDESA |
34.31 |
30.74 |
-3.57 |
| 10 |
Fernandez, Lukas J (13)
|
MDCAA |
32.93 |
32.07 |
-0.86 |
| 11 |
Selwood, Henry C (14)
|
MDCAC |
34.81 |
32.16 |
-2.65 |
| 12 |
Shaw, Rylan P (13)
|
MDROCK |
29.39 |
32.39 |
3.00 |
| 13 |
Avrunin, Brayden Z (14)
|
MDCAA |
33.07 |
32.96 |
-0.11 |
| 14 |
Suprun, Maximilian E (13)
|
MDCAA |
34.08 |
33.04 |
-1.04 |
| 15 |
Peterson, Jake (14)
|
MDCAC |
|
33.28 |
- |
| 16 |
Swanson, Benjamin H (13)
|
MDCAC |
40.81 |
33.56 |
-7.25 |
| 17 |
Mueller, Luke (13)
|
MDBAY |
30.80 |
34.69 |
3.89 |
| 18 |
Thompson, Joshua-David (13)
|
MDBAY |
43.00 |
34.73 |
-8.27 |
| 19 |
Chen, Kyle (13)
|
MDCAC |
43.90 |
34.85 |
-9.05 |
| 20 |
Metcalf, Gatlin L (13)
|
MDROCK |
29.29 |
35.03 |
5.74 |
| 21 |
Righi, Sebastian (13)
|
MDBAY |
30.70 |
35.93 |
5.23 |
| 22 |
Sasscer, Robert C (13)
|
MDCAC |
43.10 |
36.85 |
-6.25 |
| 23 |
Greene, Gavin (13)
|
MDBAY |
30.47 |
37.05 |
6.58 |
| 24 |
Lisa, Michael T (13)
|
MDROCK |
36.25 |
37.15 |
0.90 |
| 25 |
Vergara, Eduardo R (13)
|
MDCAA |
36.80 |
38.89 |
2.09 |
| 26 |
Manson, Lucas (13)
|
MDBAY |
36.38 |
40.68 |
4.30 |
| 27 |
Noltemeyer, Reed (13)
|
MDCAC |
|
48.10 |
- |
|
Miller, David (14)
|
MDBAY |
|
NS |
- |
|
Martin, Mayson W (14)
|
MDROCK |
27.99 |
NS |
- |
|
Rangaraju, Neel (14)
|
MDCAA |
42.55 |
NS |
- |